| 中文摘要 |
紫菜(Porphyra crispate)分別以熱風乾燥或冷凍燥處理後，磨粉至粒徑200 mesh萃取油脂；兩種乾燥方式之海藻萃油率(2.5%)無顯著差異，但熱風乾燥海藻萃出之藻油，其多元不飽和脂肪酸(PUFA)含量10.12 mg/dry basis，為冷凍乾燥者之82.7%，其中EPA之含量5.36 mg/dry basis僅為凍乾者之73.9%；藻油中重要之生理活性成分Sulfoquinovosylacylglycerols (SQAGs)之含量約為42 mg/g oil (4.2%)，其主要脂肪酸組成中多元不飽和脂肪酸EPA (C20:5, 30%)及花生四烯酸(C20:4, 12.7%)共佔42.7%，棕櫚酸油酸佔33.3% C16:0 ( 33.3%)、其他有油酸(C18:1 ,7.52%)及硬脂酸(C18:0, 6.83%)；藻油中SQAGs對肝癌細胞HepG2之抑制效果顯著，半抑制濃度(IC50)為125 μg/mL；直接以沙拉油萃取紫菜之乾燥粉末，可使每克沙拉油含有EPA 3.38 mg、SQAGs 0.32 mg，此方法可避免溶劑之殘留及增加植物油之保健功效；分析藻油及紅藻沙拉油，皆無反式脂肪酸(18:1 trans-9；18:2 trans-9,12)存在。利用脂氧合?之修飾可改善水產油脂中因多元不飽和脂肪酸(PUFA)之氧化而產生之魚腥味；為提高脂氧合?之儲藏安定性及重覆使用性，發展以褐藻膠包埋部分純化之脂氧合?，進行固定化，固定化酵素於4 ℃下儲藏安定性可提高16.6倍。 |
| 英文摘要 |
Fresh red algae (Porphyra crispate) were dried with hot air or frozen-dried and ground to 200 mesh prior to extract. The yields (2.5%) of algal oil of the two dried methods were not different. But the polyunsaturated fatty acids (PUFAs, 10.12 mg/dry basis) and ecicosapentaenoic acid (EPA, 5.36 mg/dry basis) contents in hot air dried algal oil are only for frozen-dried 82.7% and 73.9%, respectively. Sulfoquinovosylacylglycerols (SQAGs) separated by multiple solid-phase (SPE) from the algal oil were analyzed for fatty acid composition and the inhibition of proliferation of panel of human hepatocellular liver carcinoma cell line (HepG2) were determined. The main fatty acids in SQAGs were palmitic acid (33.3%), eicopentaenoic (C20:5, 30.0%), and arachidonic acid (C20:4, 12.7%), oleic acid (C18:1, 7.52%) and stearic acid (C18:0, 6.83%). SQAGs strongly inhibited the growth of HepG2cell line. After a 48-h continuous treatment, the IC50 value for growth inhibition was 125 μg/mL. Salad oil was instead of organic solvent to extract dried algae for avoiding the residue of organic solvent in algal oil. EPA and SQAGs in salad oil increased by 3.38 and 0.32 mg/g oil, respectively. Neither trans acids were detected in the algal oil nor in the algal salad oil. Bing algal oil contains high PUFAs that were the undesirable odor of algal oil. For reducing the undesirable odor of algal oil used lipoxygenase (LOX) to modified oil to produce desirable volatile compounds. The stability of the immobilization of LOX on alginate beads was 16.6-fold greater than that of the unbound lipoxygenase at 4 oC in 0.05 M Tris buffer (pH7.5). |