| 英文摘要 |
1. 「天然鰻苗培育技術之研究」：
The present study sampled at I-Lan,Ch-Lin,Guan-Du,Lu-Karg and Ping-Tong on April, 2006. The dominant bacteria isolated from living-feed ( red worm ) were Aeromonas hydrophila, Aeromonas spp, Enterobacteriaceae,Citrobacter spp and Flavobacterium spp.,followed by Pseudomonas spp,Edwardsiella tarda and Vibrio spp. The dominant bacteria isolated from glass eels were Aeromonas hydrophila, Aeromonas spp,Enterobacteriaceae,Citrobacter spp and Edwardsiella tarda. The dominant bacteria isolated from pond water were Aeromonas spp.,Enterobacteriaceae,Citrobacter spp, Flavobacterium spp and Pseudomonas spp.The wild caught glass eels were predominantly fed with red worms and may therefore infected by pathogenic bacteria via such a food chain. The efficacy of using Ozone 0.15ppm, ClO2 4ppm treated red worm for 30 mins and then fed glass eels could obtain better eating and survival rate. The addition of different enrichments with mixed eel meal and then fed glass eels obtained better survival rate at least 96%. The suwival rate were 95% after a lethal challenge (i.p. injection) with E. tarda .
2. 「白棘三列海膽(Tripneustes gratilla)繁養殖技術開發之研究」：
Water temperature, photoperiod and feed β-carotene level were the three factors in a four months lasted factorial experiment to investigate the growth and gonad development of the urchin. The interaction between the two factors, water temperature and photoperiod, were significantly influencing the growth and gonad formation of the urchin investigated. Body growth rate of the urchin exhibited better growth rate cultured at 25℃ than those at 29℃. Low water temperature (25℃) had been found to elevate the urchin body growth rate within each of the three different photoperiod treatments. Interaction between water temperature and photoperiod was observed by the differences in the enhancement of body growth rate from short photoperiod (10L14D) the most followed by the mid photoperiod group (12L12D) and the least enhanced the long photoperiod group (14L10D). Gonad somatic index within short photoperiod group was increased over 9% for those urchin cultured at low temperature than those at high temperature. Feed β-carotene was an independent factor which neither interacted against of temperature factor nor the factor of photoperiod on body growth rate and gonad color enhancement. However increased the level of β-carotene in feed had no effect on the improvement of GSI value.
The larval settlement experiment of the sea urchin Tripneustes gratilla had been conducted four times from March through October 2006. None of the larvae induced by various levels of MGDG and DGDG were able to settle on the surface of the Petri-dish used in the experiment. Only very few larval grew out spines during the metamorphosis stage.
It was concluded that a 60 ppm of β-carotene level cultured at 25℃ with a short photoperiod (10L14D) may speed up both the body growth rate and gonad deposit rate of the Tripneustes gratilla. |