| 英文摘要 |
Research and development of a rapid, sensitive and automatic detection platform is one of the major approaches of controlling viral diseases in aquaculture. An efficient detection platform has allowed we to monitoring the spread of important pathogens and will help to enhance the economic benefit of aquaculture industry. In this project, aiming at grouper nervous necrosis virus (NNV), attempts were made to develop an ultra-high sensitive MagnetoReduction Assay (MRA) for quantitively detect the target biomolecules (NNV viral antigens). The system shows a noise level lower than that of the magnetic signal generated by magnetic beads by 2-3 orders of magnitude. Hence, SQUID can detect a tiny change in the ac magnetic susceptibility of reagents due to the clustering or conjugation of magnetic beads associating with aquatic virus. Firstly, we will test a variety of extrinsic factors (temperature, pH, Salinity, sea water concentration, recombinant viral protein) to figure out the optimal reaction condition for NNV. Basing on the results of sensitivity and specificity assay, standard operation procedure (SOP) for NNV detection will be completed. |