| 英文摘要 |
The present study aims to determine whether zooplankton such as: rotifer (Brachionus plicatilis) and brine shrimp (artemia salina) acted as carriers of Immunoglobulin Y, which are an important role for onset feeding to marine fish culture. Mass culture of the zooplankton was carried out in sterile condition, applying uncontaminated microalgae to culture and to enrich the zooplankton. Zooplankton propagation was observed and recorded when cultured by using particular zooplankton enrichment solution. Egg yolk IgY that was produced by injecting Nervous Necrosis Virus (NNV) to a mature chicken was used for second enrichment of zooplankton. Marine larvae were fed daily with enriched zooplankton, which provided a passive immunity protection to decrease viral disease infection and increase survival rate.
Dry-up culture on agar medium can decrease the possibility of microalgae contamination. Sterilization of zooplankton was carried out by using chlorine dioxide (ClO2), which resulted in 4 to 5 ppm of chlorine dioxide that created a sterile environment and without affecting the zooplankton. Negative response was showed from commercial NNV, grouper iridovirus (GIV) diagnosis tool-kit. Rotifer propagation rate was highly acquired 96.6% by using these enrichment solution formula (fermented less-oil fish meal 10%, soybean powder 45%, fish sauce 10%, soy pomace 20%, squid paste 10%, vitamin mix 2.5%, mineral 2.5%). According to the SDS-PAGE analysis, sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that egg yolk IGY were detected from IgY-zooplankton. Results suggest that IgY-zooplankton significantly inhibited viral disease antigen from grouper (Epinepheus malabaricus) larvae. |